Isolation of anti-haptoglobin antibodies by affinity chromatography

Jerzy Osada, Wanda Dobryszycka
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引用次数: 2

Abstract

Antibodies directed against human haptoglobin type 1-1 and to porcine haptoglobin were isolated from rabbit antisera by means of affinity chromatography on Sepharose 4B. The obtained antibodies were characterized by the quantitative precipitin reaction, double immunodiffusion, immunoelectrophoresis and ultracentrifugation.

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亲和层析法分离抗触珠蛋白抗体
采用Sepharose 4B亲和层析法从兔抗血清中分离到人1-1型和猪1-1型的抗体。所得抗体经定量沉淀反应、双重免疫扩散、免疫电泳和超离心鉴定。
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