Adaptation of cells derived from human malignant tumours to growth in vitro.

J Alexander, E Bey, J M Whitcutt, J H Gear
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Abstract

Forty-five human malignant tumour specimens were cultivated in vitro in an attempt to determine the necessary conditions for tumour cell maintenance and to establish permanently-growing cell lines. Continuously-growing cultures were derived from five tumours, including carcinomas of the oesophagus and colon, a hepatoma, a mesothelioma and a retroperitoneal sarcoma. The carcinoma of the oesophagus and the hepatoma, which have adapted fully to in vitro conditions, can be regarded as established cell lines.

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人类恶性肿瘤细胞对体外生长的适应性。
在体外培养45个人类恶性肿瘤标本,试图确定肿瘤细胞维持的必要条件,并建立永久生长的细胞系。持续生长的培养物来自五种肿瘤,包括食道癌和结肠癌,肝癌,间皮瘤和腹膜后肉瘤。食管癌和肝癌已完全适应体外条件,可视为已建立的细胞系。
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