In vitro plant regeneration from leaf primordia of gum-bearing tree Aegle marmelos.

Abstract

Aegle marmelos is a middle sized slender aromatic a rmed tree native to India and distributed South-east Asia. A protocol has been developed for regeneration of plantlets from leaf primordia of Aegle marmelos. The regeneration studi es indicated that the following combinations of hormones with MS media containing K n (1.5 mg/l) + 0.5 mg/l 2, 4-D with NAA (1.0 mg/l) is most suitable for organogenic calli f ormation and shoot induction. These calli later developed shoots when transferred to MS medium cont aining NAA (1.5 mg/l) with 0.5 mg/l IBA for root initiation. Rooted plants survived well un der acclimatization.