SENSITIVITY OF MULTI-RESISTANT PSEUDOMONAS AERUGINOSA STRAINS DEPENDING ON LOCALIZATION OF INFECTIOUS PROCESS

Abstract

Objective of work was study of antibiotic resistance peculiarities and isolation localization of multi-resistant Pseudomonas aeruginosa strains. Materials and methods. The objects of investigation were multi-resistant P. aeruginosa strains isolated from pleural exudate, bronchial flushing fluid, blood, samples of wound surface and unaffected skin. Identification of the obtained cultures was carried out for morphological, tinctorial and cultural properties as well as using an automatic microbiological analyzer Vitek 32 (BioMerieux). The selected cultures were tested for sensitivity to a wide range of antibiotics using Vitek 32 (BioMerieux). Results. 18 cultures were selected among P. aeruginosa strains isolated from patients. Correspondingly to antibiotic resistance level, these strains were divided into three groups. The first group includes 7 strains (39%) with maximum resistance, representatives of the second group – 5 isolates (28%) were characterized by high resistance, and 6 cultures (33%) were referred to microorganisms with moderate resistance – the third group. Among 12 strains from the first and second groups, only two cultures were isolated in the intensive care unit. But, four cultures were isolated in the burn department, three – in orthopedics and traumatology, two – in surgery, and one – in proctology. It was found that in 50% of cases (9 strains) the cultures studied were isolated from the wound surface in patients with skin burns and after surgical intervention. At the same time, 3 strains (16% of all microorganisms) were localized in the composition of bronchial washings, as well as 2 strains (11% of all cultures) from pleural exudate and blood. From the abscess and skin of the ear, there was isolated one by one strain (6%). Conclusions. More often P. aeruginosa multi-resistant strains were isolated from the wound surface, however, the microorganisms isolated from pleural exudate were characterized the highest resistance to antibiotics, incl. to meropenem. All studied P. aeruginosa cultures retained sensitivity only to colistin and phosphomycin. The activity of other antibiotics was significantly limited, as only the netilmicin (43% of the sensitive strains), meropenem (41%), amikacin (33%), ciprofloxacin (28%) and norfloxacin (28%) influenced the isolated microorganisms.