Cardiomyopathy in vitro.

A Wada, Y Inui, H Fushimi, S Onishi
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Abstract

Phase contrast microscopy of cultured embryonic heart cells showed the beating frequency decreased more rapidly and the regularity the rhythm of of the beating cells was lost sooner in heart cells from cardiomyopathic hamsters than from the control hamsters. Studies of cultured heart cells by differential interference contrast (with Nomarski's prism) and by electron microscopy revealed a significant impediment in the maturation of the sarcomeric units in the diseased animals compared to controls. The incorporation of [14C] leucine into acid-insoluble fractions was studied, and no significant difference in incorporation between the two groups was found. An analysis of polyacrylamide gel electrophoresis revealed the possible existence of a quantitative difference in one of the composing proteins of the erythrocyte membrane between the two groups. The protein kinase activity of ghosts from the control group was more sensitive to cAMP than that from the diseased animals. In addition, the binding of [3H] cAMP to the ghost was almost identical between the two. The morphological and biochemical observations lead one to the plausible supposition that there are some differences in the interaction of the so-called catalytic and regulatory subunits between the two groups and that there is an impairment of the higher arrangement of myofibrils from their building blocks in the diseased hamster. The significance of the existence of abundant corpuscles resembling neurosecretory granules was not established by this study. They may have an etiological significance or they may be related to a disturbed function in the cultured cells of the cardiomyopathic hamster.

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体外心肌病。
培养的胚胎心脏细胞相对比显微镜显示,与对照组相比,心肌病仓鼠的心脏细胞搏动频率下降更快,搏动细胞节律的规律性丧失更快。通过差干涉对比(诺玛斯基棱镜)和电子显微镜对培养的心脏细胞的研究显示,与对照组相比,患病动物的肌体单位成熟有明显障碍。研究了[14C]亮氨酸在酸不溶性组分中的掺入情况,发现两组的掺入情况无显著差异。聚丙烯酰胺凝胶电泳分析显示,两组之间可能存在红细胞膜组成蛋白之一的数量差异。与病鼠相比,对照组鬼鼠的蛋白激酶活性对cAMP更为敏感。此外,[3H] cAMP与幽灵的结合在两者之间几乎相同。形态学和生化观察使我们有了一个合理的假设,即在两组之间所谓的催化和调节亚基的相互作用中存在一些差异,并且在患病的仓鼠中,肌原纤维的高级排列受到了损害。大量类似神经分泌颗粒的小体存在的意义尚未得到本研究的证实。它们可能具有病原学意义,也可能与心肌病仓鼠培养细胞功能紊乱有关。
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