[Opiate receptor sites in neuronal and glial cell lines in culture].

F Garcin, J Ciesielski-Treska, G Ulrich, J C Louis, P Mandel
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Abstract

A stereospecific saturable high affinity binding of 3H-naloxone has been found in 3 glial and 2 neuronal cell lines. Kinetic study of binding seems to indicate only one class of receptor sites in the 5 cell lines. Acute exposure to morphine (1 X 10(-5)M) concurrent with PGE1-induced stimulation of adenylate cyclase did not result in a decrease of the cAMP level in any cell line tested.

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[培养的神经元和神经胶质细胞系中的阿片受体位点]。
在3个胶质细胞系和2个神经元细胞系中发现了立体特异性饱和高亲和力的3h -纳洛酮结合。结合动力学研究似乎表明在5种细胞系中只有一类受体位点。急性暴露于吗啡(1 × 10(-5)M)与pge1诱导的腺苷酸环化酶刺激并没有导致任何细胞系cAMP水平的降低。
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