Genetic manipulation in higher organisms. I. Goldfish ova as materials of operation, mRNA mediated alteration of the liver specific isozymes.

Scientia Sinica Pub Date : 1977-11-01
M C Niu, T C Tung
{"title":"Genetic manipulation in higher organisms. I. Goldfish ova as materials of operation, mRNA mediated alteration of the liver specific isozymes.","authors":"M C Niu,&nbsp;T C Tung","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Both crucian and carp egg-mRNAs were found to mediate the transformation of goldfish tail from the double (veil-shaped) to the single (fork-shaped). Livers of these single tailed goldfish were tested for genetic change using lactate dehydrogenase isozymes (LDH) as marker. Electrophoresis of liver extracts on starch gel showed that the liver specific LDH-C4 isozymes of both the egg-mRNA and liver-mRNA injected goldfish resembled the control goldfish except an additional intermediate band. The migration distance of this new band matched the third band of the hybrids (carp X goldfish). These findings lead to the conclusion that carp egg-mRNA contains a fraction partly responsible for liver development. Rat liver was found possessing the cathodally migrating C4 isozymes. Injection of rat liver-mRNA into goldfish eggs resulted in the intensification of the liver specific C4 isozymes. It appears therefore that the input informational molecules (liver-mRNA) and the output molecular products (liver C4 isozymes) are causally related. This causal relationship would pave the way for genetic manipulation in higher organisms.</p>","PeriodicalId":21694,"journal":{"name":"Scientia Sinica","volume":"20 6","pages":"803-6"},"PeriodicalIF":0.0000,"publicationDate":"1977-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Both crucian and carp egg-mRNAs were found to mediate the transformation of goldfish tail from the double (veil-shaped) to the single (fork-shaped). Livers of these single tailed goldfish were tested for genetic change using lactate dehydrogenase isozymes (LDH) as marker. Electrophoresis of liver extracts on starch gel showed that the liver specific LDH-C4 isozymes of both the egg-mRNA and liver-mRNA injected goldfish resembled the control goldfish except an additional intermediate band. The migration distance of this new band matched the third band of the hybrids (carp X goldfish). These findings lead to the conclusion that carp egg-mRNA contains a fraction partly responsible for liver development. Rat liver was found possessing the cathodally migrating C4 isozymes. Injection of rat liver-mRNA into goldfish eggs resulted in the intensification of the liver specific C4 isozymes. It appears therefore that the input informational molecules (liver-mRNA) and the output molecular products (liver C4 isozymes) are causally related. This causal relationship would pave the way for genetic manipulation in higher organisms.

分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
高等生物的基因操纵。1 .金鱼卵作为手术材料,mRNA介导肝脏特异性同工酶的改变。
发现鲫鱼和鲤鱼卵mrna都介导了金鱼尾巴从双尾(面纱状)到单尾(叉状)的转变。以乳酸脱氢酶同工酶(LDH)为标记,检测单尾金鱼肝脏的遗传变化。肝提取物淀粉凝胶电泳结果显示,除多了一条中间带外,注射了卵mrna和肝脏mrna的金鱼肝脏特异性LDH-C4同工酶与对照金鱼相似。新条带的迁移距离与杂种(鲤鱼X金鱼)的第三条带相匹配。这些发现可以得出结论,鲤鱼卵mrna中含有部分对肝脏发育起部分作用的成分。发现大鼠肝脏具有阴极迁移的C4同工酶。将大鼠肝脏mrna注射到金鱼卵中,导致肝脏特异性C4同工酶的增强。因此,输入信息分子(肝脏mrna)和输出分子产物(肝脏C4同工酶)似乎是因果相关的。这种因果关系将为高等生物的基因操纵铺平道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
The nature of radiation damage of haemopoietic stem cells under continuous irradiation at low dose rate. [3H-Methyl]-methionine as possible methyl donor for formation of 5'-terminus of in vitro synthesized mRNA of cytoplasmic polyhedrosis virus of silkworm Bombyx mori. The "bolus flow" solution of the plasma between the red blood cells flowing through a capillary. Studies on developmental biology of embryogenesis in higher plants--effect of actinomycin D and cycloheximide on the formation of peroxidase during rice and embryogenesis and germination. The gene frequency of serum albumin variants in Chinese and the electrophoretic characterization of several serum albumin variants.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1