Diagnosis of anaerobic infections by gas-liquid chromatography.

C E Nord
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Abstract

Anaerobic bacteriology of clinical specimens is an expensive and time-consuming operation. In the present investigation it was shown that fatty acids produced by anaerobic bacteria may serve as bacterial markers in clinical material. Ten specimens of pus were analyzed by gas liquid chromatography and the results were compared with the bacteriological findings. Good correlations were found for the recovery of Bacteroides fragilis and the presence of isobutyric, butyric and succinic acids. It is concluded that direct gas-liquid chromatography of clinical specimens provides a rapid presumptive test for the presence of B. fragilis in anaerobic infections.

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厌氧感染的气液色谱诊断。
临床标本的厌氧细菌学是一项昂贵且耗时的工作。本研究表明,厌氧菌产生的脂肪酸可作为临床材料中的细菌标记物。采用气液色谱法对10份脓液进行分析,并与细菌学检查结果进行比较。发现脆弱拟杆菌的回收率与异丁酸、丁酸和琥珀酸的存在有良好的相关性。结论:临床标本直接气液色谱法可快速推测易碎芽孢杆菌在厌氧感染中的存在。
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