Multiplex PCR CMOS Biochip for Detection of Upper Respiratory Pathogens including SARS-CoV-2

Abstract

A 1024-pixel CMOS biochip for multiplex polymerase chain reaction application is presented. Biosensing pixels include 137dB DDR photosensors and an integrated emission filter with OD~6 to perform real-time fluorescence-based measurements while thermocycling the reaction chamber with heating and cooling rates of > ±10°C/s. The surface of the CMOS IC is biofunctionalized with DNA capturing probes. The biochip is integrated into a fluidic consumable enabling loading of extracted nucleic acid samples and the detection of upper respiratory pathogens, including SARS-CoV-2.