Structure prediction and analysis of mouse amiloride-sensitive cation channel 2, neuronal using bioinformatics tools

Abstract

This Acid-sensing ion channels (ASICs) are voltage-insensitive cation channels expressed in both central and peripheral neurons. ASICs are activated by extracellular protons, and several agents modify the response. ASICs are members of the larger degenerin/epithelial Na_channel (DEG/ENaC) family of ion channels. The sequence of mouse amiloride-sensitive cation channel 2, neuronal, ACCN2_MOUSE has been analyzed by different bioinformatics tools in order to get the primary, secondary structure and 3D structure. The sequence of ACCN2_MOUSE was analyzed through Protparam in order to find the physical and chemical properties. The transmembrane helices, coiled coils are predicted using Bioinformatics tools. Comparative modeling has been performed in order to get the 3D structure of ACCN2_MOUSE. Our structure prediction was based on the availability of the 3D model of the homologous protein from Amiloride-sensitive cation channel 2, neuronal chicken (2QTSE). This will be useful for further protein-protein interaction prediction, protein-protein docking, molecular docking and pharmacological studies.