Generation of rotational flow in microfluidics device for formation of spheroid

Abstract

Spheroid is formed by making it agitate and circle in cell suspension. However, the injection of the cells used as pieces of spheroids, the recovery at the time of tissue formation, exchange of a culture medium, etc. are performed manually, and it is hard to say that efficient organization construction is performed. Thus, we suggest utility and possibility of applying microfluidics for formation of toroidal-like spheroid. Microfluidic devices to generate rotational flow were fabricated, and we conducted experiments using mouse embryo fibroblast cell.