Web-based Computational Tools for Calculating Optimal Testing Pool Size for Diagnostic Tests of Infectious Diseases

Abstract

Pooling together samples and testing the resulting mixture is gaining considerable interest as a potential method to markedly increase the rate of testing for SARS-CoV-2, given the resource limited conditions. Such pooling can also be employed for carrying out large scale diagnostic testing of other infectious diseases, especially when the available resources are limited. Therefore, it has become important to design a user-friendly tool to assist clinicians and policy makers, to determine optimal testing pool and sub-pool sizes for their specific scenarios. We have developed such a tool; the calculator web application is available at https://riteshsingh.github.io/poolsize/. The algorithms employed are described and analyzed in this paper, and their application to other scientific fields is also discussed. We find that pooling always reduces the expected number of tests in all the conditions, at the cost of test sensitivity. The No sub-pooling optimal pool size calculator will be the most widely applicable one, because limitations of sample quantity will restrict sub-pooling in most conditions.