The Effect of Fermentation Time on Product Quality of Starfruit Juice Probiotic Drinks with Starter Culture L. plantarum B1765

Nur Islahah, P. R. Wikandari
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Abstract

This study aims to determine the effect of fermentation time on microbiological (total lactic acid bacteria), chemistry (pH, total titrated acid) and organoleptic (color, aroma, and flavor) qualities of the product of the starfruit (Averrhoa carambola L.) probiotic juice drink cultured by L. plantarum B1765. L. plantarum B1765 had been studied has potency as probiotics candidate. The fermentation time used is 0, 6, 18, and 24 hours. Total lactic acid bacteria (LAB) are tested by the total plate count (TPC) method, pH tested with a pH meter, total titrable acidity (TTA) tested with acid-base titration, and organoleptic tested by the hedonic scale method. The length time of fermentation affects the total lactic acid bacteria (LAB), pH, and TTA and shows a significant difference up to 18 hours of fermentation. Total BAL increased from 1.08 × 108 to 4.20 × 108 CFU/ml. pH dropped from 3.80 to 3.18. TTA increased from 0.44% to 0.81%. The organoleptic test shows the level of preference for the color, aroma, and flavor at the best fermentation time for 18 hours with a successive value of 3.20; 3.20; and 3.30 with the like category. The starfruit juice fermented by L. plantarum B1765 is potencial as a probiotic agent drink.
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发酵时间对植物乳杆菌B1765发酵剂杨桃汁益生菌饮料产品品质的影响
本研究旨在确定发酵时间对L. plantarum B1765培养的杨桃(Averrhoa carambola L.)益生菌果汁饮料产品的微生物(总乳酸菌)、化学(pH、总滴定酸)和感官(色、香、味)品质的影响。植物乳杆菌B1765已被研究为潜在的益生菌。发酵时间为0、6、18、24小时。总乳酸菌(LAB)用总平板计数(TPC)法测定,pH值用pH计测定,总可滴定酸度(TTA)用酸碱滴定法测定,感受器用hedonic scale法测定。发酵时间长短影响总乳酸菌(LAB)、pH和TTA,发酵18小时后差异显著。总BAL由1.08 × 108 CFU/ml增加至4.20 × 108 CFU/ml。pH值从3.80下降到3.18。TTA从0.44%增加到0.81%。感官测试表明,最佳发酵时间为18小时时,对色、香、味的偏好水平连续值为3.20;3.20;类似类别的是3.30。植物乳杆菌B1765发酵的杨桃汁具有开发益生菌饮料的潜力。
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