Induksi Mutasi Kromosom Dengan Iradiasi Sinar Gamma Cobalt 60 Untuk Merakit Padi (Oryza sativa) Tahan Kekeringan Secara In Vitro

Indah Permata Dewi
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Abstract

A lot of people in the world choose rice as their main source of carbohydrate beside maize and wheat. The increasing of world population make increasing of the rice  demand. Increasing of the rice demand doesn’t followed by the rice supply.  In order to solve that problem, researcher try to find a new paddy cultivar which is can be planted in dry area. This research aims to study the osmotic pressure that still can be accepted by paddy var. Sintanur,  to study LD50 (lethal dose 50) value in the paddy especially in Sintanur and to study the interaction between irradiation and the osmotic pressure. In this research, paddy var. Sintanur is irradiated by gamma ray of Cobalt60 at six dosages 0 Gy, 100 Gy, 200 Gy, 300 Gy, 400 Gy, 500 Gy. Then each irradiated seed is planted at four kind of mediums that contains Polyethylene glycol (PEG) at four levels of concentration are I0 (0 g/l PEG), I1 (116,538 g/l PEG), I2 (174,6 g/l PEG) and I3 (219,547 g/l PEG). Based on the data analyzed, the highest PEG concentration for drought tolerant selection in paddy var. Sintanur is 174,674 g/l PEG. There is an interaction between irradiation and PEG medium that influencing plant height and shoot multiplication. LD 50 (lethal dose 50) of paddy var. Sintanur is 375 Gy.   Keyword: paddy, Sintanur, cobalt60, drought tolerant, irradiation
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用钴射线60来诱导染色体突变,用于在体外制造耐旱米粒(Oryza sativa)
除了玉米和小麦,世界上很多人选择大米作为碳水化合物的主要来源。世界人口的增加使大米需求量增加。大米需求的增加并不会带来大米供应的增加。为了解决这一问题,研究人员试图寻找一种可以在干旱地区种植的水稻新品种。本研究旨在研究Sintanur水稻品种仍能接受的渗透压,研究水稻特别是Sintanur水稻的LD50(致死剂量50)值,研究辐照与渗透压的相互作用。在本研究中,水稻品种Sintanur被钴60 γ射线照射,照射剂量为0 Gy、100 Gy、200 Gy、300 Gy、400 Gy和500 Gy。然后将每颗辐照过的种子种在四种含有聚乙二醇(PEG)的培养基中,浓度分别为I0 (0 g/l PEG)、I1 (116,538 g/l PEG)、I2 (174,6 g/l PEG)和I3 (219,547 g/l PEG)。结果表明,水稻品种Sintanur的最高PEG浓度为174,674 g/l。辐照与PEG介质之间存在相互作用,影响株高和茎部增殖。水稻品种Sintanur的致死剂量50为375 Gy。关键词:水稻,Sintanur,钴60,耐旱,辐照
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