EFEK PROBIOTIK Bacillus megaterium PTB 1.4 DAN Pediococcus pentosaceus E2211 TERHADAP REPONS IMUN DAN KELANGSUNGAN HIDUP IKAN LELE (Clarias sp.) SELAMA UJI TANTANG Aeromonas hydrophila

Abstract

probiotik respons A. hydrophila . Total hemoglobin, hematokrit, total leukosit, (P<0,05) meningkat ke-35 ke-40. Kelangsungan lele berhasil dipertahankan 87,62±4,83%. Total bakteri A. hydrophila meningkat hari ke-35 dan menurun pada hari ke-40. Pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 pada dosis 0,5% dapat menekan pertumbuhan bakteri A. hydrophila dan meningkatkan status kesehatan ikan lele. Abstract Bacterial diseases in aquaculture activities has become a problem often faced by farmers. One type of bacteria that causes disease in catfish ( Clarias sp.) is Aeromonas hydrophila bacteria. The aim of this study that to evaluate the effect of probiotics Bacillus megaterium PTB 1.4 and Pediococcus pentosaceus E2211 on the immune response and survival of catfish during a challenge test with A. hyrophila bacteria. The probiotics used were combination of 0.5% B. megaterium PTB 1.4 and 0.5% P. pentosaceus E2211. Provision of probiotics was given on day-0 to day-30 through feed. The challenge test was carried out on day 30 to day 40. The results showed that the administration of probiotics could affect the immune response and survival rate of catfish in suppressing A. hydrophila infection. Total erythrocytes, hemoglobin, hematocrit, total leukocytes, phagocytic activity and lysozyme activity were significant (P<0.05) increasing on day-35 and decreasing on day-40. The survival rate of catfish was successfully maintained at 87.62±4.83%. Total A. hydrophila bacteria increased on the day-35 and decreased on the day-40. Administration of probiotics B. megaterium PTB 1.4 and P. pentosaceus E2211 at a dose of 0.5% can suppress the growth of A. hydrophila bacteria and improve the health status of catfish.