STUDY ON POTENTIAL ENGINEERING OF ESCHERICHIA COLI XANTHOSE PHOSPHORYLASE STRAIN-PRODUCER

Abstract

Genetic engineering is an actively developing branch of modern biotechnology. Using the methods of genetic engineering, it is possible to construct new forms of microorganisms that can synthesize a variety of substances, including enzymes. Xanthosine phosphorylase is the second purine nucleoside phosphorylase (PNP-II) in E. coli. This enzyme performs both reactions of phosphorolysis and the synthesis of purine deoxy / ribonucleosides. Due to this ability, xanthosine phosphorylase can catalyze the reaction of the formation of nicotinamide riboside. This substance is a precursor of the most important coenzyme NAD+ in the body, which plays a key role in the aging process. As a result of the study, a new strain of E. coli pET42a-xapA was constructed. This strain produces the protein xanthosine phosphorylase.