G. Heder, W. Jakob, W. Halle, B. Mauersberger, G. Kambach, K.D. Jentzsch, P. Oehme
{"title":"Influence of porcine corpus luteum extract on DNA synthesis and proliferation of cultivated fibroblasts and endothelial cells","authors":"G. Heder, W. Jakob, W. Halle, B. Mauersberger, G. Kambach, K.D. Jentzsch, P. Oehme","doi":"10.1016/S0014-4908(79)80069-1","DOIUrl":null,"url":null,"abstract":"<div><p>The angiogenic activity in corpus luteum (corpus luteum angiogenesis factor = CLAF) reported first by us in 1977 was enriched by ammonium sulfate precipitation and further purified by ion exchange chromatography. Extracts producedin this way from corpora lutea of cyclus synchronized pigs have proven effective to stimulate DNA synthesis and proliferation of both calf aorta endothelial cells and primary mouse embryo fibroblasts cultivated either under optimal or suboptimal (depletion) conditions. The mitogenic action of these extracts has been found to be dose-dependent and similar to that of bovine brain extract produced according to the preparation method published by <span>Gospodarowicz</span>.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"17 7","pages":"Pages 493-497"},"PeriodicalIF":0.0000,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(79)80069-1","citationCount":"19","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimentelle Pathologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014490879800691","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 19
Abstract
The angiogenic activity in corpus luteum (corpus luteum angiogenesis factor = CLAF) reported first by us in 1977 was enriched by ammonium sulfate precipitation and further purified by ion exchange chromatography. Extracts producedin this way from corpora lutea of cyclus synchronized pigs have proven effective to stimulate DNA synthesis and proliferation of both calf aorta endothelial cells and primary mouse embryo fibroblasts cultivated either under optimal or suboptimal (depletion) conditions. The mitogenic action of these extracts has been found to be dose-dependent and similar to that of bovine brain extract produced according to the preparation method published by Gospodarowicz.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
