[Electron microscopical and autoradiographical studies on the so-called yolk-nuclei in the oocytes of Nemacheilus barbatulus (L.) and Phoxinus phoxinus (L.) (Pisces, Teleostei) (author's transl)].

Abstract

The yolk-nuclei in the oocytes of the teleosts Noemacheilus barbatulus (L.) and Phoxinus phoxinus (L.) were investigated by electron microscopy, autoradiography and ultrahistochemistry. Yolk-nuclei were only found in stage I. They consist of mitochondria, an osmiophilic material and in Noemacheilus partly of dictyosomes. It was demonstrated by marking with [3H]-uridine, that the osmiophilic material contains RNA. The osmiophilic material gets into the juxtanuclear cytoplasm. There it is surrounded by mitochondria. The oocytes of Noemacheilus and Phoxinus contain up to 40 yolk-nuclei, which are only visible by electron microscopy. The yolk-nuclei of both species dissolve in early stage II. Their components disperse in the cytoplasm.