{"title":"Isolation of carcinoembryonic antigen by an improved procedure","authors":"David G. Pritchard , Marianne L. Egan","doi":"10.1016/0161-5890(78)90135-9","DOIUrl":null,"url":null,"abstract":"<div><p>An improved procedure for the isolation of carcinoembryonic antigen (CEA) has been developed. Lengthy dialysis, ultrafiltration and lyophilization steps following perchloric acid extraction are replaced by a simple ethanol precipitation of the CEA prior to the Chromatographie purification steps. Chemical and immunochemical characterization of CEA prepared by this procedure showed no significant differences compared to CEA prepared by the more lengthy procedure not using ethanol precipitation.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90135-9","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078901359","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8
Abstract
An improved procedure for the isolation of carcinoembryonic antigen (CEA) has been developed. Lengthy dialysis, ultrafiltration and lyophilization steps following perchloric acid extraction are replaced by a simple ethanol precipitation of the CEA prior to the Chromatographie purification steps. Chemical and immunochemical characterization of CEA prepared by this procedure showed no significant differences compared to CEA prepared by the more lengthy procedure not using ethanol precipitation.
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