{"title":"On the immune precipitation of phosphorylcholine lipids with tepc 15 mouse myeloma protein and with anti-lecithin sera from guinea pigs","authors":"Barbara Niedieck, Ursula Kuck, Hedda Gardemin","doi":"10.1016/0161-5890(78)90076-7","DOIUrl":null,"url":null,"abstract":"<div><p>The BALB/c mouse myeloma protein TEPC 15 (T 15) and sera of guinea pigs immunized with lecithin (lec) precipitate in agarose gels with mixed micelles bearing the phosphorylcholine lipids (PC-lipids) lec, lysolecithin and sphingomyelin. The T 15 IgA and the guinea pig anti-lee antibody are both totally inhibited in agarose gels containing phosphorylcholine. The advantages of defined PC-lipids in contrast to the commonly employed complex antigens for the detection of anti-PC immunoglobulins are discussed.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90076-7","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078900767","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
Abstract
The BALB/c mouse myeloma protein TEPC 15 (T 15) and sera of guinea pigs immunized with lecithin (lec) precipitate in agarose gels with mixed micelles bearing the phosphorylcholine lipids (PC-lipids) lec, lysolecithin and sphingomyelin. The T 15 IgA and the guinea pig anti-lee antibody are both totally inhibited in agarose gels containing phosphorylcholine. The advantages of defined PC-lipids in contrast to the commonly employed complex antigens for the detection of anti-PC immunoglobulins are discussed.
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