D Banauch, W Brümmer, W Ebeling, H Metz, H Rindfrey, H Lang, K Leybold, W Rick, H J Staudinger
{"title":"[A glucose dehydrogenase for the determination of glucose concentrations in body fluids (author's transl)].","authors":"D Banauch, W Brümmer, W Ebeling, H Metz, H Rindfrey, H Lang, K Leybold, W Rick, H J Staudinger","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The isolation of glucose dehydrogenase from Bacillus megaterium M 1286 is outlined. Data on the specificity of the enzyme towards carbohydrates are given. A specific method for glucose determination using this enzyme was developed. Methods and results of four variants of this glucose determination are presented: End point determination in the UV range, determination with formazan as reaction product, kinetic determination in the UV range, and continuous flow analysis in the UV range (AutoAnalyzer method).</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1975-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur klinische Chemie und klinische Biochemie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The isolation of glucose dehydrogenase from Bacillus megaterium M 1286 is outlined. Data on the specificity of the enzyme towards carbohydrates are given. A specific method for glucose determination using this enzyme was developed. Methods and results of four variants of this glucose determination are presented: End point determination in the UV range, determination with formazan as reaction product, kinetic determination in the UV range, and continuous flow analysis in the UV range (AutoAnalyzer method).