Detection of circulating immune complexes in human sera by simplified assays with polyethylene glycol

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of immunological methods Pub Date : 1977-06-01 DOI:10.1016/0022-1759(77)90051-5

M. Digeon, M. Laver, J. Riza, J.F. Bach

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引用次数: 282

Abstract

The search for circulating immune complexes by precipitation tests using polyethylene glycol (PEG) was performed on a series of normal and pathological sera. Various factors affecting PEG precipitation were studied. Immunoglobulins and complement factors precipitated by PEG (3.5%) were quantified and their significance was discussed in relation to serum levels. The PEG test was compared to labeled C1q binding test with a fairly good correlation. The direct evaluation of the amount of C4 precipitated with IgG by 3% PEG (C4 test) provided a simpler routine assay than the C1q binding test for detecting complement-fixing immune complexes. The direct PEG test and the C4 test gave positive results in patients with diseases generally presumed to be associated with immune complexes including systemic lupus erythematosus, acute glomerulonephritis, bacterialsub-acute endocarditis and chronic active hepatitis. The demonstration of HBs antigen and antibody after acid dissociation of PEG precipitates from heptitis B seronegative sera illustrated the fact that PEG does precipitate and thus concentrates circulating immune complexes.

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聚乙二醇简化法检测人血清循环免疫复合物

用聚乙二醇(PEG)沉淀试验对一系列正常和病理血清进行循环免疫复合物的搜索。研究了影响聚乙二醇沉淀的各种因素。定量测定PEG沉淀的免疫球蛋白和补体因子(3.5%)，并讨论其与血清水平的关系。PEG试验与标记C1q结合试验比较，具有较好的相关性。用3% PEG (C4试验)直接评价IgG沉淀C4的量，为检测补体固定免疫复合物提供了比C1q结合试验更简单的常规方法。一般认为与免疫复合物相关的疾病，包括系统性红斑狼疮、急性肾小球肾炎、细菌性亚急性心内膜炎和慢性活动性肝炎，直接PEG试验和C4试验结果均为阳性。从乙型肝炎血清阴性血清中分离聚乙二醇沉淀物后，HBs抗原和抗体的证明表明，聚乙二醇确实沉淀，从而集中了循环免疫复合物。

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来源期刊

Journal of immunological methods 医学-免疫学

CiteScore

4.10

自引率

0.00%

发文量

120

审稿时长

3 months

期刊介绍： The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens. (2) Purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies. (5) Localizing antigens and/or antibodies in tissues and cells. (6) Detecting, and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Documenting cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. (12) Molecular techniques for studying immune cells and their receptors. (13) Imaging of the immune system. (14) Methods for production or their fragments in eukaryotic and prokaryotic cells. In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods. In general these manuscripts are by solicitation however anyone interested in submitting a review can contact the Reviews Editor and provide an outline of the proposed review.