Reconciling differences between tumor oxygenation measurements performed using /sup 19/F NMR spectroscopy and imaging of perfluorocarbon (PFC) emulsions

Abstract

The purpose of this study was to investigate methods for reconciling differences between tumor oxygenation measurements performed using /sup 19/F NMR spectroscopy and imaging of sequestered PFC's. In vivo data from RIF-1 tumor bearing C/sub 3/H mice were acquired using /sup 19/F NMR spectroscopy and imaging, and the imaging data were analyzed via three different methods: (1) average of the T/sub 1/ map; (2) weighted average of the T/sub 1/ map which compensated for the spin density (M/sub 0/); and (3) region of interest (ROI) analysis of the tumor region. It was shown that compensating for M/sub 0/ in the imaging maps allowed correction of much of the discrepancy between tumor oxygenation values that were calculated from imaging and spectroscopic data.