[Polyacrylamide gel electrophoresis of Theragra chalcogramma and bovine opsins in the presence of sodium dodecyl sulphate].

Abstract

Fish and bovine opsins were found generally in monomeric forms with slight content of dimers at electrophoresis in 1% sodium dodecyl sulphate--0.005 M sodium phosphate buffer (system I). Opsins have a high tendency to oligomerization and formation of trimers and higher oligomers in 1+5 M urea (system II). The molecular weight of nonomers and dimers of opsins in system I calculated according to the standard proteins, increased with a rise of acrylamide concentration. The authors believe that in both systems the hydrophobic interactions that not the reactions of intermolecular S-S bonds formation are supposed to be on the basis of opsins oligomerization.