A. L. Gronlund, J. Dickinson, P. Kille, J. Harwood, R. Herbert, D. Francis, H. Rogers
{"title":"Plant WEE1 Kinase Interacts with a 14-3-3 Protein, GF14ω but a Mutation of WEE1 at S485 Alters Their Spatial Interaction","authors":"A. L. Gronlund, J. Dickinson, P. Kille, J. Harwood, R. Herbert, D. Francis, H. Rogers","doi":"10.2174/1874294700903010040","DOIUrl":null,"url":null,"abstract":"In animals, 14-3-3 proteins bind two cell cycle proteins WEE1 and CDC25 stabilising their phosphorylated \nstate. We report here for the first time interactions between WEE1 and 14-3-3 proteins both in vitro and in vivo in plants. \nThe Arabidopsis 14-3-3 family partitions into either an Epsilon or Non-Epsilon group. In a yeast 2-hybrid screen \nArabidopsis WEE1 interacted with the Non-Epsilon group. Subsequently, we focussed on Non-Epsilon GF14","PeriodicalId":355995,"journal":{"name":"The Open Plant Science Journal","volume":"38 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2009-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"12","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Plant Science Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874294700903010040","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12
Abstract
In animals, 14-3-3 proteins bind two cell cycle proteins WEE1 and CDC25 stabilising their phosphorylated
state. We report here for the first time interactions between WEE1 and 14-3-3 proteins both in vitro and in vivo in plants.
The Arabidopsis 14-3-3 family partitions into either an Epsilon or Non-Epsilon group. In a yeast 2-hybrid screen
Arabidopsis WEE1 interacted with the Non-Epsilon group. Subsequently, we focussed on Non-Epsilon GF14