Genotoxicity of esentai and ulken almaty rivers water on animal test-systems

A. B. Karazhanova, N. M. Kerimbek, A. Lovinskaya, S. Kolumbayeva, S. Abilev
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Abstract

Ecological monitoring, including water resources, is included in a set of measures for the rational use and protection of the environment. Currently, the assessment of water resources’ genotoxicity and mutagenicity is an essential component of ecological monitoring. This research aimed to investigate water’s genotoxicity from the Esentai and Ulken Almaty rivers flowing in Almaty. It was established that the content of Fe, Cu, Co, Ni, Pb, and Cd in the Almaty rivers water does not exceed the maximum permissible concentration (MPC). In contrast, zinc content in the Esentai and Ulken Almaty rivers’ water exceeded the MPC, respectively, by 1.1 and 7.7-folds, manganese – 2.1, and 1.9-folds. It was found that water samples from the Esentai and Ulken Almaty rivers showed a DNA-damaging effect on the studied cells (bone marrow, liver, kidneys, and spleen) of laboratory mice. DNA damage was determined using the Comet assay. In the cells of the studied organs in the experimental groups, the frequency of single-strand DNA breaks a statistically significant increase compared to the control group’s animal. The genotoxic action’s organospecificity of the studied river waters on laboratory mice was established. According to their sensitivity to water’s genotoxic effect, experimental mice’s organs can arrange in the following order: spleen and bone marrow> liver> kidneys. An increase in lipid peroxidation products was found in the laboratory mice’ liver that drank water from the Esentai and Ulken Almaty rivers. Thus, the results of the physicochemical, molecular-genetic, and biochemical analysis of water on animal test objects indicate the presence in the investigated natural surface waters of chemicals with genotoxic and toxic activity. окисления липидов в печени лабораторных мышей, принимавших воду из рек Есентай и Улкен Алматы. Таким образом, результаты физико-химического, молекулярно-генетического и биохимического анализа воды на животных тест-объектах обладающих Ключевые слова: поверхностные тяжелые металлы, метод ДНК-комет, органоспе ци-фичность, перекисное окисление липидов.
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阿拉木图埃森泰河和乌尔肯河水在动物试验系统上的遗传毒性
包括水资源在内的生态监测是一套合理利用和保护环境的措施。目前,水资源遗传毒性和诱变性评价是生态监测的重要组成部分。本研究旨在调查阿拉木图的埃森泰河和乌尔肯河的水的遗传毒性。确定阿拉木图河水中Fe、Cu、Co、Ni、Pb、Cd的含量不超过最大允许浓度(MPC)。相比之下,埃森泰河和乌尔肯-阿拉木图河的锌含量分别超过了MPC的1.1倍和7.7倍,锰含量分别超过了2.1倍和1.9倍。研究发现,埃森泰河和乌尔肯-阿拉木图河的水样对实验小鼠的研究细胞(骨髓、肝脏、肾脏和脾脏)产生了dna损伤作用。DNA损伤用Comet测定法测定。在实验组所研究器官的细胞中,单链DNA断裂的频率与对照组的动物相比有统计学上的显著增加。建立了所研究河水对实验小鼠的遗传毒性作用的器官特异性。根据小鼠对水的基因毒性作用的敏感性,实验小鼠的脏器可按以下顺序排列:脾、骨髓、>、肝脏、>、肾脏。在饮用埃森泰河和乌尔肯-阿拉木图河水的实验小鼠肝脏中发现脂质过氧化产物增加。因此,对动物试验对象的水进行物理化学、分子遗传学和生化分析的结果表明,在所调查的自然地表水中存在具有遗传毒性和毒性活性的化学物质。окислениялипидоввпеченилабораторныхмышей,принимавшихводуизрекЕсентайиУлкенАлматы。Такимобразом,результатыфизико——химического,молекулярно——генетическогоибиохимическогоанализаводынаживотныхтест——объектахобладающихКлючевыеслова:поверхностныетяжелыеметаллы,методДНК-комет,органоспеци-фичность,перекисноеокислениелипидов。
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