The Usefulness of Calretinin Immunohistochemistry for Rectal Suction Biopsy to Diagnose Hirschsprung’s Disease

Jae-Gyu Jeong, Sang Pyo Kim, Eunyoung Jung, Soon-Ok Choi
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Abstract

Purpose: Hirschsprung’s disease (HD) is a congenital intestinal disorder with absence of ganglion cells in the intestinal muscle and submucosa. Diagnosis is based on histopathological study such as H&E, and acetylcholinesterase (AchE) immunohistochemistry. Calretinin immunohistochemistry was introduced as a new diagnostic method against limitations of other staining. The aim of this study is to investigate the usefulness of calretinin immunohistochemistry for the diagnosis of HD compared to H&E and AchE. Methods: Ten patients with HD and 22 non-HD patients were included in the study. H&E staining, AchE and calretinin immunohistochemistry were performed in all 32 patients. All slides were evaluated by same single pathologist and the diagnostic value was calculated for each H&E stain, AchE immunohistochemical staining, and calretinin immunohistochemical staining. Results: Calretinin method had sensitivity of 100% and specificity of 100% for diagnosis of HD. Its diagnostic accuracy was 100%. AchE staining showed 100% of specificity and 80% of sensitivity. Diagnostic accuracy of H&E staining was 56.3%. Conclusion: We concluded that calretinin immunohistochemistry is a very useful and valuable method to diagnosis HD patient.
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Calretinin免疫组化对直肠抽吸活检诊断巨结肠病的价值
目的:巨结肠病(HD)是一种先天性肠道疾病,在肠道肌肉和粘膜下层缺乏神经节细胞。诊断是基于组织病理学研究,如H&E和乙酰胆碱酯酶(AchE)免疫组织化学。Calretinin免疫组化是一种新的诊断方法,克服了其他染色方法的局限性。本研究的目的是探讨calretinin免疫组织化学与H&E和AchE相比对HD诊断的有用性。方法:选取10例HD患者和22例非HD患者作为研究对象。32例患者均行H&E染色、AchE、calretinin免疫组化。所有载玻片均由同一位病理学家评估,并计算每次H&E染色、AchE免疫组织化学染色和calretinin免疫组织化学染色的诊断价值。结果:Calretinin法诊断HD的敏感性为100%,特异性为100%。其诊断准确率为100%。AchE染色特异性100%,敏感性80%。H&E染色诊断正确率为56.3%。结论:calretinin免疫组化对HD患者的诊断是一种非常有用和有价值的方法。
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