Antibacterial and Cytotoxicity Analyses on Aloe vera Body Soap

A. Lo, Nurizzati Mohd Daud, N. M. Zain
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Abstract

Aloe vera is a well-known succulent plant and it is often used to moisturise and sooth the skin. In this study, Aloe vera is incorporated into natural soap to investigate the antibacterial properties and cytotoxicity of the samples. The antibacterial properties of the Aloe vera sample was determined by performing zone inhibition and bacterial colony test on Escherichia coli (E. coli). The cell viability test was conducted with human skin fibroblast cells through MTT assay. The Aloe vera samples showed antibacterial properties through the presence of inhibition zones and in the bacterial colony analysis, the Aloe vera samples have inhibitory effect against E. coli with a bacterial retardation percentage of 31.97±0.90%. The cell viability percentage of 95.30±11.16% was found on the Aloe vera sample with normal cell morphology. These results testified that the Aloe vera sample was biocompatible and possessed antibacterial properties which is suitable to be incorporated in skin products.
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芦荟沐浴皂的抗菌及细胞毒性分析
芦荟是一种众所周知的多肉植物,它经常被用来滋润和舒缓皮肤。在本研究中,芦荟被掺入天然肥皂,以研究样品的抗菌性能和细胞毒性。对大肠杆菌进行区抑菌和菌落试验,测定芦荟样品的抑菌性能。采用MTT法对人皮肤成纤维细胞进行细胞活力测定。结果表明,芦荟样品存在抑菌带,对大肠杆菌有抑菌作用,抑菌率为31.97±0.90%。细胞形态正常的芦荟细胞存活率为95.30±11.16%。结果表明,该芦荟样品具有良好的生物相容性和抗菌性能,适合应用于护肤品中。
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