Photosensitive pigments formed with rat opsin.

Investigative ophthalmology Pub Date : 1976-10-01
R Crouch
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Abstract

Photosensitive pigments are formed in vitro from rat opsin with the rods in suspension or as digitonin solutions. Rhodopsin, isorhodopsin I, and isorhodopsin II were generated with 11-cis, 9-cis and 9, 13-dicis retinal, respectively. An isorhodopsin I analogue was formed on combination with 9-cis 13-desmethyl-14-methylretinal. The absorption spectra and photosensitivities of these pigments were found to be similar to the corresponding bovine pigments although the rates of formation are several fold slower.

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由大鼠视蛋白形成的光敏色素。
光敏色素是由大鼠视蛋白与视杆在悬浮液或洋地黄苷溶液中形成的。分别以11-顺式、9-顺式和9,13 -盘视网膜生成视紫红质、异视紫红质I和异视紫红质II。异紫质1类似物与9-顺式13-去甲基-14-甲基视网膜结合形成。发现这些色素的吸收光谱和光敏性与相应的牛色素相似,尽管形成速度慢了几倍。
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