Identification of Chicken Eimeria Species from Microscopic Images by Using MLP Deep Learning Algorithm

Mucahit Buyukyilmaz, Ali Osman Çibikdiken, M. A. Abdalla, H. Seker
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引用次数: 5

Abstract

Eimeria has more than one species of every single genus of animals that causes diseases that may spread at fast speed and therefore adversely affects animal productivities and results in animal death. It is therefore essential to detect the disease and prevent its spread at the earliest stage. There have been some attempts to address this problem through the analysis of microscopic images. However, due to the complexity, diversity, and similarity of the types of the species, there need more sophisticated methods to be adapted for the intelligent and automated analysis of their microscopic images by using machine- learning methods. To tackle this problem, a deep-learning-based architecture has been proposed and successfully adapted in this study where Chicken fecal microscopy images have been analyzed to identify nine types of these species. The methodology developed includes two main parts, namely (i) pre-processing steps include the techniques that convert image into gray level, extract cell walls, remove background, rotate cell to vertically aligned position and move to their center and (ii) MLP-based deep learning technique to learn features and classify the images, for which Keras model was utilized. Based on the outcome of a 5-fold cross validation that was repeated for 100 times, the approach taken has yielded an average accuracy of 83.75%±0.60, which is comparable to the existing methods.
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基于MLP深度学习算法的鸡艾美耳球虫显微图像识别
艾美耳球虫在每一个动物属中都有一个以上的物种,这些物种引起的疾病可能迅速传播,从而对动物生产力产生不利影响并导致动物死亡。因此,必须在最早阶段发现该病并防止其传播。已经有一些尝试通过分析显微图像来解决这个问题。然而,由于物种类型的复杂性、多样性和相似性,需要更复杂的方法来适应使用机器学习方法对其显微图像进行智能和自动化分析。为了解决这个问题,研究人员提出了一种基于深度学习的架构,并在本研究中成功地采用了这种架构,分析了鸡粪便显微镜图像,以识别出这些物种的九种类型。所开发的方法包括两个主要部分,即(i)预处理步骤包括将图像转换为灰度,提取细胞壁,去除背景,将细胞旋转到垂直对齐位置并移动到其中心的技术;(ii)基于mlp的深度学习技术,以学习特征并对图像进行分类,其中使用了Keras模型。基于重复100次的5重交叉验证结果,所采用的方法的平均准确率为83.75%±0.60,与现有方法相当。
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