In-channel modifications for improved optical detection of phosphate in a microfluidic chip design

Joyce O'Grady, Nigel J. Kent, I. Maguire, P. McGinnity, E. Jennings, F. Regan
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引用次数: 1

Abstract

Recent developments in the area of low cost optical analysers has enabled rapid, reliable and robust analysis of water nutrient levels, such as phosphate, in water systems. Herein, describes an enhancement study of a previously demonstrated lab- on-a-disc (LOAD) centrifugal microfluidic device for the detection of phosphate in freshwater. The LOAD device utilizes a microfluidic sample processing to enable high precision metering and reagent mixing, followed by colorimetric analysis (at 880 nm) of the resultant complex. A customisable and complementary, in-house analysis system was also developed to enhance user interaction and enable rapid analysis. This analysis system delivers both disc centrifugation and automated colourimetric detection of the LOAD device, with recording of data transmitted via PC interface. The aim of this study is to maintain the same level of sensitivity of the current[1] system with a reduced pathlength. The limit of detection (LOD) and limit of quantification (LOQ) for this new revised system are as follows: The blackened chip obtained the best sensitivity with an LOD and LOQ of 6 and 19 μg L−1 respectively, followed by the P80 roughened chip which achieved 13 and 38 μg L−1, these results will be discussed further in this paper.The previously demonstrated microfluidic platform demonstrated an optical path length detection of 75 mm for optimal detectability, resulting in low quantity of sample testing per disc. This work details the optimisation of the disc design through a reduction of path length, therefore increasing the number of test replications on-disc by two-fold and a reduction in reagent consumption, whilst maintaining the same sensitivity using photo-enhancement techniques. The photo-enhancement techniques described in this paper utilize detection channel surface modifications, e.g. coatings and refractive index modification. These assist in significantly improving the signal-to-noise (S/N) with increased transmittance, hence increasing the overall sensitivity that can be achieved using the system. This piece of work focuses on the optical improvement using surface modification strategies in detection enhancement.
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微流控芯片设计中改进磷酸盐光学检测的通道内改进
低成本光学分析仪领域的最新发展使水系统中磷酸盐等水营养水平的快速,可靠和强大的分析成为可能。本文描述了先前演示的用于检测淡水中磷酸盐的盘上实验室(LOAD)离心微流控装置的增强研究。LOAD设备利用微流体样品处理实现高精度计量和试剂混合,然后对所得复合物进行比色分析(880 nm)。此外,还开发了一个可定制和互补的内部分析系统,以加强用户交互和实现快速分析。该分析系统提供圆盘离心和自动比色检测LOAD设备,并通过PC接口传输数据记录。本研究的目的是在减少路径长度的情况下保持电流[1]系统的相同灵敏度水平。新体系的检出限(LOD)和定量限(LOQ)如下:黑化芯片的灵敏度最高,LOD和LOQ分别为6和19 μg L−1,P80粗化芯片次之,LOD和LOQ分别为13和38 μg L−1,本文将进一步讨论这些结果。先前演示的微流控平台展示了75毫米的光路长度检测,以获得最佳的可检测性,从而降低了每个圆盘的样品测试量。这项工作详细介绍了通过减少路径长度来优化光盘设计,从而将光盘上的测试重复次数增加两倍,并减少试剂消耗,同时使用光增强技术保持相同的灵敏度。本文描述的光增强技术利用探测通道表面修饰,例如涂层和折射率修饰。这有助于显著提高信噪比(S/N),增加透光率,从而提高使用系统可以实现的整体灵敏度。这项工作的重点是在检测增强中使用表面修饰策略进行光学改进。
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