TBC Bacteria Detection in Microscopic Image With Watershed Countur Method

Rodan Hilmi Dawwas
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Abstract

Tuberculosis (TB) is an infectious disease that can be detected using a sputum sample. TB cases in Indonesia have spread throughout the region; the highest cases are in West Java. This problem makes the government do some handling and prevention of TB disease. The Bandung City Health Office (DKKB) conducted a cross-test to diagnose TB using a sputum sample. So in this study, a TB bacteria detection system, namely Mycobacterium Tuberculosis (MTB), will be made in sputum samples and their number to diagnose TB. Detection and calculation of the number of MTB are done by processing the image on the sputum sample using the watershed contour detection method. In this study, sputum sample data were obtained from DKKB. The acquisition of microscopic images at each point of the field of view is carried out using an SLR camera connected directly to the microscope to replace the function of the ocular lens. In this study, the microscopic sputum sample images were classified into positive and negative using the watershed and colorspace methods and were tested on a total of 90 microscopic images. From the system testing results, the system accuracy level is 100%, and the system precision is 100% for the detection of TB diagnosis. The system processing time averaged 5.811 seconds for 90 images used.
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分水岭计数法在显微图像中检测TBC细菌
结核病(TB)是一种可以通过痰样本检测到的传染病。印度尼西亚的结核病病例已蔓延到整个区域;最高的病例在西爪哇。这一问题促使政府采取一些措施来处理和预防结核病。万隆市卫生局(DKKB)进行了一项使用痰样本诊断结核病的交叉试验。因此,本研究将对痰液样本及其数量进行结核分枝杆菌(Mycobacterium Tuberculosis, MTB)检测,以诊断结核。采用分水岭轮廓检测方法对痰液样本上的图像进行处理,完成MTB数目的检测和计算。在本研究中,痰样本数据来自DKKB。使用直接连接到显微镜的单反相机来代替目镜的功能,在视场的每个点上进行显微图像的采集。本研究采用分水岭法和色彩空间法对痰样显微镜图像进行阳性和阴性分类,并对共90张显微镜图像进行检测。从系统测试结果来看,该系统的准确率水平为100%,对于结核病的检测诊断,该系统的准确率为100%。对于使用的90个图像,系统处理时间平均为5.811秒。
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