{"title":"Non-contact measurement of oxygen consumption rate of single oocyte using stripe-shaped fluorescence sensor on microfluidic chip","authors":"Masanobu Kito, H. Maruyama, F. Arai","doi":"10.1109/MHS.2014.7006137","DOIUrl":null,"url":null,"abstract":"We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS). Oxygen around the oocyte can be spherically diffused because of high oxygen permeability of PDMS and the stripe pattern of sensor. This sensor achieves two-dimensional measurement of OCR by image processing. We demonstrated non-contact measurement of OCR of single oocyte on a microfluidic chip.","PeriodicalId":181514,"journal":{"name":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","volume":"56 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2014 International Symposium on Micro-NanoMechatronics and Human Science (MHS)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/MHS.2014.7006137","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS). Oxygen around the oocyte can be spherically diffused because of high oxygen permeability of PDMS and the stripe pattern of sensor. This sensor achieves two-dimensional measurement of OCR by image processing. We demonstrated non-contact measurement of OCR of single oocyte on a microfluidic chip.
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