Adhesion of C. albicans to Acrylic Resin Containing Fluoridated Apatite-Coated TiO2

Abstract

Purpose: This study investigated the inhibitory effect of acrylic resin incorporating FAp-TiO2 against the adhesion of C. albicans.Materials and methods: FAp-TiO2 was added at 1, 5, 10 wt% to acrylic resin to form specimen disks of 7 mm diameter, and the surface roughness was measured. Then, C. albicans was cultured aerobically at 37 °C for 24 hours in glucose peptone yeast extract (GPY) broth, and adjusted to OD=1 at 550 nm with a KCl-buffer. The disks were immersed in the C. albicans suspension for 2 hours at 37 °C with UVA from a black light source. After the incubation, the disks were washed gently with phosphate buffer saline (PBS), and the luminescent signal derived from the viable cells on the disks was determined using a bioluminescence adenosine triphosphate (ATP) assay.Results: In the measurement of surface roughness, no significant difference was observed between any of the specimens. The adhesion assay of C. albicans revealed that a statistically significant decrease was observed in FAp-TiO2 when compared with the control (p‹0.01).Conclusion: We suggest that acrylic resin incorporating FAp-TiO2 prevents the adhesion of C. albicans, indicating a wide range of possibilities for the practical use of new photocatalysts in acrylic resin.