Biocatalyzed Hydrolysis of Residual Oils and Proteins from Flax and Camelina Oilseed Press Cakes Using Lipase and Protease

Abstract

In this study, a PoC (Proof of Concept) of a possible biomass valorization of flax and camelina oilseed press cakes was presented. Biocatalyzed hydrolysis of residual oils and proteins extracted from these wastes was studied. The biotransformation of oils was performed using commercial immobilized lipases, including Amano PS, Amano AK, and Candida Antarctica Lipase B (CALB). Acylglycerols were partially or fully hydrolyzed using Amano PS and AK. Triglycerides were not hydrolyzed by CALB, which behaved differently. Enzymatic hydrolysis of the proteins extracted from these cakes was performed using commercial proteases, including Amano Protease P and Amano Protease M. This was the first study to quantify the amino acids in the reaction products. The results were also compared to the hydrolysates obtained using 6 M HCl. Some differences were observed in the amino acid profiles depending on the enzyme used and the protein sample.