L. M. Ramos-Ponce, J. Contreras-Esquivel, J. Saenz, G. Lara-Cisneros, Y. Garza-García
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引用次数: 4
Abstract
Mycophenolic acid (MPA) can be produced from Penicillium pinophilum by submerged fermentation. Here we reported the use of the Plackett-Burman design to screen the limiting components for MPA production and subsequent use the response surface methodology was employed to derive a statistical model for media optimization towards production of MPA. The results obtained of Plackett-Burman indicated limitations of three components in the basal medium, aspartic acid, methionine and Tween 80. The determinant coefficient was 0.80, ensuring a satisfactory adjustment of the model to the experimental values. This statistical design was good in improving the titer of MPA up 923 mg/L and a specific productivity of 0.05 mg AMP/mg of biomass/h after 10 day of fermentation.
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