Preparation and optimization of aminolevulinic acid with gold nanoparticles for photothermal and photodynamic therapies applications

Abstract

The use of gold nanoparticles (AuNps) as the vehicle for 5-Aminolevulinic acid (ALA) delivery for photodynamic and photothermic plasmonic therapies is a promising approach, especially with the recent demonstration that this photosensitizer immobilization on the particle surface improves reactive oxygen species (ROS) formation, increasing its cytotoxicity. Gold nanorods (AuNRs) present an absorption spectrum shifted to 700 nm, within the tissue transparency window, which allows excitation of the nanoparticles situated deeper in the tissues. Here, we describe a new synthesis method that was applied to control the shape of the gold nanoparticles during its synthesis. To obtain ALA:AuNRs, precursor ALA:AuNps were irradiated by ultrashort laser pulses. The variation of the laser parameters such as pulse energy and duration and irradiation time was assessed. The relevant mechanisms are discussed.