{"title":"Binding interactions of progesterone and other C21 steroids with rat uterine cytosol.","authors":"B A Booth, A E Colás","doi":"10.1159/000301523","DOIUrl":null,"url":null,"abstract":"<p><p>The binding of progesterone and other C21 steroids by rat uterine cytosol preparations was studied by equilibrium dialysis. Scatchard plots and preliminary computer-assisted analysis indicated at least two types of binding sites for progestrone. Individual Scatchard plots for cortisol and corticosterone binding were linear with Ka's 0.4 and 1.4 times 10(9) M-1, respectively. Inhibition analyses and modeling of competition experiments suggest, however, that the situation is more complex. There was no binding of labeled dexamethasone.</p>","PeriodicalId":75889,"journal":{"name":"Gynecologic investigation","volume":"6 5","pages":"272-84"},"PeriodicalIF":0.0000,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000301523","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gynecologic investigation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000301523","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11
Abstract
The binding of progesterone and other C21 steroids by rat uterine cytosol preparations was studied by equilibrium dialysis. Scatchard plots and preliminary computer-assisted analysis indicated at least two types of binding sites for progestrone. Individual Scatchard plots for cortisol and corticosterone binding were linear with Ka's 0.4 and 1.4 times 10(9) M-1, respectively. Inhibition analyses and modeling of competition experiments suggest, however, that the situation is more complex. There was no binding of labeled dexamethasone.
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