Multi-Dimensional Microscopic Imaging of Biological Tissues Based on Polarization Spectrum

Abstract

In this study, we build an integrated microscope with multi-spectrum polarization imaging (MSPI) technology. Polarization imaging is achieved by rotating the polarizer and quarter wave plate, which can obtain parametric images of Stokes parameters, the polarization phase retardance, and the polarization ellipticity orientation angle. Hyperspectral imaging is achieved by using an electronically controlled liquid crystal wavelength filter in front of the broad-band visible light source. By calculating and integrating the intensity and parametric images under different illumination spectrum, the limitation of current polarization imaging due to the wavelength-dependent absorption of the sample was avoided. A significant improvement in resolving power and contrast was found in the imaging results of oocyte cells.