Liang Gao, Wenjia Bai, Lei Jin, P. Xue, Hui Ma, Dieyan Chen
{"title":"Two-photon excited fluorescence imaging of cell spindles for developmental biology","authors":"Liang Gao, Wenjia Bai, Lei Jin, P. Xue, Hui Ma, Dieyan Chen","doi":"10.1117/12.741582","DOIUrl":null,"url":null,"abstract":"By integrating two-photon excited fluorescence (TPEF) technique into confocal optical system, TPEF images of zygote spindles of ICR (Internal Control Region) rats have been obtained in high resolution for the first time. Experimental results demonstrate the validity of TPEF as an efficient probe in investigating cell spindles at early embryonic developmental stage.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"15 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Conference on Photonics and Imaging in Biology and Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1117/12.741582","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
By integrating two-photon excited fluorescence (TPEF) technique into confocal optical system, TPEF images of zygote spindles of ICR (Internal Control Region) rats have been obtained in high resolution for the first time. Experimental results demonstrate the validity of TPEF as an efficient probe in investigating cell spindles at early embryonic developmental stage.