Clinical Evaluation of the Multiplex PCR Assay for the Detection of Bacterial Pathogens in Respiratory Specimens from Patients with Pneumonia

Chae Lim Jung, Mi Ae Lee, W. Chung
{"title":"Clinical Evaluation of the Multiplex PCR Assay for the Detection of Bacterial Pathogens in Respiratory Specimens from Patients with Pneumonia","authors":"Chae Lim Jung, Mi Ae Lee, W. Chung","doi":"10.5145/KJCM.2010.13.1.40","DOIUrl":null,"url":null,"abstract":"Background: Community-acquired pneumonia (CAP) is a major infectious disease with significant morbidity and mortality worldwide. Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis are common pathogens of CAP; however, the conventional methods used to detect these agents, including culturing, lack sensitivity and are time-consuming. We evaluated a recently developed multiplex PCR assay which can test these agents simultaneously. Methods: One hundred patients with pneumonia and 99 healthy adults were tested using the Seeplex Pneumobacter ACE Detection assay (Seegene, Inc., Seoul, Korea). Culture and urinary antigen tests were also performed. Results: In patients with pneumonia, the positive detection rates of PCR for S. pneumoniae and H. influenzae were 52.0% (52/100) and 30.0% (30/100), respectively, those of M. pneumoniae and L. pneumophila were 2.0% (2/100) and 1.0% (1/100), respectively, and B. pertussis and C. pneumoniae were not detected. In healthy adults, the detection rates of S. pneumoniae and H. influenzae revealed similar results, 53.5% (53/101) and 40.4% (40/101), respectively, and the other four pathogens were not detected. The sensitivity and specificity of PCR for S. pneumoniae in pneumonia patients were 100% (95% confidence interval [CI], 87.9∼100%) and 65.7% (95% CI, 55.2∼76.5%), respectively, according to the urinary antigen test and cultures of the respiratory samples and blood. Conclusion: Differentiating S. pneumoniae and H. influenzae colonization from infection was difficult using the PCR assay. Therefore, the use of this assay is inappropriate for the diagnosis of pneumonia due to these agents, although multiplex PCR assay would be useful for the detection of M. pneumoniae and L. pneumophila. (Korean J Clin Microbiol 2010;13:40-46)","PeriodicalId":143093,"journal":{"name":"Korean Journal of Clinical Microbiology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Clinical Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5145/KJCM.2010.13.1.40","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7

Abstract

Background: Community-acquired pneumonia (CAP) is a major infectious disease with significant morbidity and mortality worldwide. Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis are common pathogens of CAP; however, the conventional methods used to detect these agents, including culturing, lack sensitivity and are time-consuming. We evaluated a recently developed multiplex PCR assay which can test these agents simultaneously. Methods: One hundred patients with pneumonia and 99 healthy adults were tested using the Seeplex Pneumobacter ACE Detection assay (Seegene, Inc., Seoul, Korea). Culture and urinary antigen tests were also performed. Results: In patients with pneumonia, the positive detection rates of PCR for S. pneumoniae and H. influenzae were 52.0% (52/100) and 30.0% (30/100), respectively, those of M. pneumoniae and L. pneumophila were 2.0% (2/100) and 1.0% (1/100), respectively, and B. pertussis and C. pneumoniae were not detected. In healthy adults, the detection rates of S. pneumoniae and H. influenzae revealed similar results, 53.5% (53/101) and 40.4% (40/101), respectively, and the other four pathogens were not detected. The sensitivity and specificity of PCR for S. pneumoniae in pneumonia patients were 100% (95% confidence interval [CI], 87.9∼100%) and 65.7% (95% CI, 55.2∼76.5%), respectively, according to the urinary antigen test and cultures of the respiratory samples and blood. Conclusion: Differentiating S. pneumoniae and H. influenzae colonization from infection was difficult using the PCR assay. Therefore, the use of this assay is inappropriate for the diagnosis of pneumonia due to these agents, although multiplex PCR assay would be useful for the detection of M. pneumoniae and L. pneumophila. (Korean J Clin Microbiol 2010;13:40-46)
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
多重PCR检测肺炎患者呼吸道病原菌的临床评价
背景:社区获得性肺炎(CAP)是世界范围内发病率和死亡率较高的主要传染病。肺炎链球菌、流感嗜血杆菌、肺炎支原体、肺炎衣原体、嗜肺军团菌和百日咳博德泰拉是CAP常见的病原体;然而,用于检测这些试剂的传统方法,包括培养,缺乏灵敏度且耗时。我们评估了最近开发的多重PCR检测,可以同时检测这些试剂。方法:采用Seegene, Inc., Seoul, Korea的Seeplex肺杆菌ACE检测法对100例肺炎患者和99名健康成人进行检测。还进行了培养和尿抗原检测。结果:肺炎患者中,肺炎链球菌和流感嗜血杆菌的PCR检出率分别为52.0%(52/100)和30.0%(30/100),肺炎支原体和嗜肺乳杆菌的PCR检出率分别为2.0%(2/100)和1.0%(1/100),百日咳双歧杆菌和肺炎支原体未检出。在健康成人中,肺炎链球菌和流感嗜血杆菌的检出率相似,分别为53.5%(53/101)和40.4%(40/101),其余4种病原体未检出。根据尿抗原检测和呼吸道样本及血液培养结果,PCR检测肺炎链球菌的敏感性和特异性分别为100%(95%可信区间[CI], 87.9 ~ 100%)和65.7% (95% CI, 55.2 ~ 76.5%)。结论:用PCR方法区分肺炎链球菌和流感嗜血杆菌的定植与感染是困难的。因此,虽然多重PCR检测可用于检测肺炎支原体和嗜肺乳杆菌,但这种检测方法不适用于这些药物引起的肺炎的诊断。(中华临床微生物学杂志2010;13:40-46)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Diversity of Integrons Carrying blaVIM-2 Cassette in Pseudomonas spp. and Acinetobacter spp. A Case of Diabetic Foot Ulcer Caused by Arcanobacterium haemolyticum and Streptococcus agalactiae Haemophilus parainfluenzae Infective Endocarditis Confirmed by 16S rRNA Sequence Analysis from Culture Negative Tissue An Unusual Feature of Malaria: Exflagellated Microgametes of Malarial Parasites in Human Peripheral Blood Lung Abscess and Bacteremia Caused by Neisseria flavescens and Streptococcus sanguis in Patient with Idiopathic Hypereosinophilic Syndrome
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1