Uncertainty Evaluation and Analysis of Enterobacteriaceae Count in Proficiency Testing

Xiu-ting Chang, Sui-shan Yang, Xiao-ju Luo, Zhu Meng, Li-dan Kou, Zuo-rong Xie
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Abstract

: Objective: To analyze and evaluate the uncertainty the results of the proficiency testing (ACAS-PT 1083 (2021)) of enterobacteriaceae count. Methods: According to the operational instructions of capability verification and GB 4789.41-2016 National food safety standard-Food microbiology test-Enterobacteriaceae count, the enterobacteriaceae of the capability verification sample were tested quantitatively. The suspected colonies were identified by VITEK2. The uncertainty of test results was assessed in accordance with JJF 1059.1-2012 and GB/T 27418-2017 Evaluation and expression of measurement uncertainty. Results: The test result of sample 21-Q997 was 4.2 ×10 3 CFU/mL. The test results were satisfactory as the Z values of the proficiency test was -0.8 (|Z|
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熟练度检验中肠杆菌科计数的不确定度评定与分析
目的:对肠杆菌科细菌计数能力检验(ACAS-PT 1083(2021))结果的不确定度进行分析和评价。方法:根据能力验证操作指导书和GB 4789.41-2016食品安全国家标准《食品微生物学试验-肠杆菌科计数》,对能力验证样品的肠杆菌科进行定量检测。用VITEK2对可疑菌落进行鉴定。试验结果的不确定度按照JJF 1059.1-2012和GB/T 27418-2017《测量不确定度评定与表达》进行评定。结果:样品21-Q997的检测结果为4.2 ×10 3 CFU/mL。测试结果令人满意,熟练度测试Z值为-0.8 (|Z|)
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