Modern technologies of microbiological research in ophthalmology. part 1

Abstract

Purpose. To highlight modern microbiological technologies for the identification of microorganisms as: mass spectrometry, polymerase chain reaction (PCR), sequencing, laser light scattering. Material and methods. To carry out the review, a search was carried out for scientific publications of domestic and foreign authors on the resources PubMed, Medline, eLIBRARY from 2008 to 2021, devoted to the currently existing methods of identifying microorganisms with an emphasis on ophthalmological microbiological diagnostics. Results. Modern microbiological technologies for the identification of microorganisms are able to assess not only the presence of the pathogen, but also to record its viability, the degree of virulence, currently implemented on the basis of modern technologies: mass spectrometry, polymerase chain reaction (PCR), sequencing and others. Conclusion. The microbiological technologies presented in the review make it possible to ensure high speed, productivity, sufficient sensitivity and economic availability of microbiological analysis, and are able to increase the probability of a positive outcome of the therapy. Key words: identification of microorganisms, polymerase chain reaction, sequencing, mass spectrometry