Gold nanorods for paper-based genetic assays

C. Borri, S. Centi, S. Chioccioli, P. Bogani, F. Micheletti, Marco Gai, P. Grandi, S. Laschi, Francesco Tona, A. Barucci, N. Zoppetti, R. Pini, F. Ratto
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Abstract

Owing to their unique photophysical properties and ease of synthesis and functionalization, gold nanoparticles represent an excellent choice to develop colorimetric paper-based biosensors. Here, we selected anisotropic gold nanorods for their outstanding multiplexability and intrinsic brightness with respect to usual gold nanospheres. In particular, we compared two prominent strategies of functionalization with oligonucleotides to detect a specific DNA target, i.e. direct thiolation with a mercapto-probe, or amidation with an amino-probe in association with a carboxy-cross-linker. We found that the former provides much more consistent results, at the expense of greater complexity.
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用于纸基遗传分析的金纳米棒
由于其独特的光物理性质和易于合成和功能化,金纳米颗粒是开发比色纸基生物传感器的绝佳选择。在这里,我们选择了各向异性金纳米棒,因为它们具有出色的可复用性和相对于普通金纳米球的固有亮度。特别是,我们比较了两种突出的寡核苷酸功能化策略来检测特定的DNA靶标,即用巯基探针直接硫代化,或用氨基探针与羧基交联剂结合进行酰胺化。我们发现前者提供了更加一致的结果,但代价是更大的复杂性。
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