Hydroxyapatite Particulate Nanofiber Modified Titanium: In-Vitro Bioactivity

Abstract

Current work demonstrates the ability of titanium based implant surfaces to promote human osteoblasts (HOBs) differentiation and matrix production, and enhance osseointegration in vitro. Titanium surface was modified by electrospinning with sol-gel-derived hydroxyapatite (HAp) and successively calcined at various temperatures. After heat-treatment, the crystal structure of the filmed titanium oxide and sol-gel-derived crystalline HAp on titanium’s surface was identified using wide-angle X-ray diffraction. Surfaces of three different samples, HAp electrospun and calcined at 600, 700 and 800 °C, were investigated in terms of their ability of promotion, adherence, proliferation and differentiation of human HOB cells in vitro up to 6 days. The cells cultured on electrospun and 800 °C calcined titanium surfaces showed the best results among three samples in terms of adhesion, growth and proliferation of HOBs. This work would provide a promising alternative for titaniumbased medical devices since it provides enhancement both on the surface and bulk properties.