{"title":"Validation of a high-performance liquid chromatographic method for the simultaneous assessment of vitamin D isoforms","authors":"Z. Duma, L. Bekker, A. Khine","doi":"10.36303/jmlstsa.2020.2.2.36","DOIUrl":null,"url":null,"abstract":"of [OH] discussion: Good linearity was achieved for 25 OHD2, 25 OHD3 and 24,25 (OH)2D with r 2 ≥ 0.99, with limits of detection ≤ 5.0 ng/ml and lower limits of quantitation ≤ 10.0 ng/m. The 1,25 (OH) 2 D metabolites, present in the blood at pg/ml levels, could not be quantified within the required ranges. Comparing total 25 OH Vit D immunoassay and HPLC results showed a poor correlation (r 2 = 0.073), possibly due to cross-reactivity of the antibodies in the immunoassay with other Vit D isoforms. Conclusion: This method provides a precise and accurate, with a potential for high throughput, determination of Vit D isoforms in the routine laboratory.","PeriodicalId":286949,"journal":{"name":"The Journal of Medical Laboratory Science and Technology of South Africa","volume":"5 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Medical Laboratory Science and Technology of South Africa","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36303/jmlstsa.2020.2.2.36","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
of [OH] discussion: Good linearity was achieved for 25 OHD2, 25 OHD3 and 24,25 (OH)2D with r 2 ≥ 0.99, with limits of detection ≤ 5.0 ng/ml and lower limits of quantitation ≤ 10.0 ng/m. The 1,25 (OH) 2 D metabolites, present in the blood at pg/ml levels, could not be quantified within the required ranges. Comparing total 25 OH Vit D immunoassay and HPLC results showed a poor correlation (r 2 = 0.073), possibly due to cross-reactivity of the antibodies in the immunoassay with other Vit D isoforms. Conclusion: This method provides a precise and accurate, with a potential for high throughput, determination of Vit D isoforms in the routine laboratory.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
