{"title":"The directions and methods of genetic engineering and cultivation of lactic acid bacteria for the beginners","authors":"K. Okano","doi":"10.4109/jslab.30.8","DOIUrl":null,"url":null,"abstract":"Genetic engineering of lactic acid bacteria is a difficult task for the beginners due to large difference in strains of interest and poor access for genetic tools. In this review, based on the author’ s experiences, experimental directions and methods for selecting the host strain and vectors; transformation of lactic acid bacteria; optimization of expression system; gene disruption/insertion; cultivation of recombinants","PeriodicalId":117947,"journal":{"name":"Japanese Journal of Lactic Acid Bacteria","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Lactic Acid Bacteria","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4109/jslab.30.8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Genetic engineering of lactic acid bacteria is a difficult task for the beginners due to large difference in strains of interest and poor access for genetic tools. In this review, based on the author’ s experiences, experimental directions and methods for selecting the host strain and vectors; transformation of lactic acid bacteria; optimization of expression system; gene disruption/insertion; cultivation of recombinants
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