Atatürk Baraj Gölü’nde (Türkiye) Yaşayan Carassius gibelio (Bloch, 1782) türünün mtDNA COI ve cyt b Analizi

Abstract

Objective: The aim of this study is to compare the C. gibelio species living in Atatürk Dam Lake with the sequences in the gene bank based on the sequences of the mtDNA COI and Cyt b gene regions and to perform phylogenetic analyzes. Material and Methods: Individuals of the target species living in the Bozova region of Atatürk Dam Lake were randomly selected from fishermen and purchased. Total DNA isolation was performed from muscle tissue using the GeneJET Genomic DNA Purification Kit (Thermo Scientific) according to the protocol instructions. Then, using specific primers of mtDNA COI and Cyt-b regions, the relevant regions were amplified by PCR method. The obtained PCR products were carried out in Agarose gel and the samples forming the product were selected and sent to the commercial firm as service procurement and sequence analysis was performed with the 3500 XL Genetic Analyzer device. Results: Sequences of 620 and 580 bp in length were obtained for the mtDNA COI and cyt b gene regions, respectively. Although the same haplotype was detected for the mtDNA COI region with individuals living in Turkey before, a new haplotype was detected for the mtDNA cyt b gene region that was not previously defined. Conclusion: Sequence analyzes of mtDNA COI and cyt b gene regions of individuals belonging to the C. gibelio species living in Atatürk Dam Lake were performed for the first time in this study and compared with the data in the gene bank.