Temperature-Annealing-Cycling Hole Burning Spectroscopies of Dye and Dye-Oligonucleotide Doped in Polymer Films

Ta-Chau Chang, C. Chiang, S. Chou
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Abstract

We have measured the temperature-annealing-cycling hole spectra of 5-(and 6-) carboxy-X-rhodamine N-hydroxy succinimidyl ester (RhSE) and the RhSE molecule with binding to the oligonucleotide of ten bases of Guanine (RhSE-10G) doped in polyvinyl alcohol (PVOH) films, respectively. Figure 1 shows the temperature-annealing-cycling hole spectra of zero phonon hole (ZPH) of RhSE/PVOH and RhSE-10G/PVOH, which were taken after burning 5 and 10 mins with a flux of ~ 40 mW/cm2 at λB ~ 5812 Å, respectively. It is found that the holes are reduced by 40 % and 50 % in the annealing spectra at Ta ~ 18 K. However, part of the holes are recovered when the systems are cycled back to the burning temperature at Tc = 6 K. It is found that the hole reduction in the annealing spectrum of RhSE-10G/PVOH is more dominant than that of RhSE/PVOH.
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聚合物薄膜中掺杂染料和染料寡核苷酸的温度退火循环烧孔光谱
在聚乙烯醇(PVOH)薄膜中分别测量了5-(和6-)羧基- x -罗丹明n -羟基琥珀酰氨基酯(RhSE)和与鸟嘌呤10个碱基寡核苷酸结合的RhSE分子(RhSE- 10g)的温度-退火-循环空穴光谱。图1为RhSE/PVOH和RhSE- 10g /PVOH的零声子空穴(ZPH)在λB ~ 5812 Å下,以~ 40 mW/cm2的通量分别燃烧5 min和10 min后的温度退火循环空穴谱图。在Ta ~ 18k的退火光谱中,孔洞分别减少了40%和50%。然而,当系统循环回到Tc = 6 K的燃烧温度时,部分孔洞被恢复。结果表明,与RhSE/PVOH相比,RhSE- 10g /PVOH退火谱中的空穴还原更为明显。
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