Viral haemorrhagic disease of rabbit: purification and characterization of a strain isolated in France.

G Le Gall, E Boilletot, J P Morisse
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Abstract

The causative agent of rabbit viral haemorrhagic disease (VHD) was purified by CsCl density gradient centrifugation from liver homogenates of rabbits infected with a strain of VHD virus isolated in France. Electron microscopy observations revealed that the virions were icosahedral with a diameter of 33-35 nm, and with an average buoyant density of 1.33. Structural proteins were investigated by SDS-polyacrylamide gel electrophoresis and western blotting. The results indicated the presence of a major structural protein of 64 kDa and other minor proteins of molecular weight of 43, 36 and 32 kDa, the last presumably resulting from the proteolytic degradation of VP 64. These results correspond with the description made by the different authors who identify the VHD virus as a member of the Caliciviridae family.

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兔病毒性出血性疾病:法国分离株的纯化和特性。
用CsCl密度梯度离心法从法国分离的一株兔病毒性出血病(VHD)病毒感染的兔肝脏匀浆中纯化出致病菌。电镜观察显示,病毒粒子为二十面体,直径为33 ~ 35 nm,平均浮力密度为1.33。采用sds -聚丙烯酰胺凝胶电泳和western blotting检测结构蛋白。结果表明,存在一个64 kDa的主要结构蛋白和其他分子量为43、36和32 kDa的次要结构蛋白,后者可能是vp64的蛋白水解降解所致。这些结果与将VHD病毒确定为冠状病毒科成员的不同作者所做的描述相一致。
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